Steganography

First things first, always use binwalk or foremost to isolate files from any other embedded stuff.
And of course use strings (ASCII, UTF8, UTF16) before anything advanced.

Images

Image manipulation in Python

Pixel color inverting example:

png_invert.py
import Image
if __name__ == '__main__':
	img = Image.open('input.png')
	in_pixels = list(img.getdata())
 	out_pixels = list()
 
	for i in range(len(in_pixels)):
		r = in_pixels[i][0]
		g = in_pixels[i][1]
		b = in_pixels[i][2]
		out_pixels.append( (255-r, 255-g, 255-b) )
 
	out_img = Image.new(img.mode, img.size)
	out_img.putdata(out_pixels)
	out_img.save("output_inverted.png", "PNG")

Audio

Video/Animation